Anatomical Studies and Evaluation of Genetic Stability in Plantlets Derived from Somatic Embryos of Arabica Coffee

Anatomical characteristics regenerant plantlet of Arabica coffee (Coffea arabica L.) were observed to determine the difference performance between Sigararutang and Maragogige grown in shooting rooting medium. Transverse sections fresh roots, stems leaves three-month-old plantlets from somatic embryos collected used for study. Maragogipe as materials chosen based on bean size origin. Stomata microscopically abaxial leaf paradermal section. A conformity test compare parent plant was perform genetic stability. Assessment stability measured by using sequence trnL (UAA) region. The result showed that all anatomical have a greater number than (root diameter, cortex thickness, distance long stele, short endodermis stem maximum stele minimum epidermis diameter stomatal closing, length total density, adaxial midrib vascular bundles, lamina thickness), except aperture, opening, opening density. Taxonomists may be able use these anatomic traits supplementary proof determination coffee. Molecular analysis there genetically identical organisms plant. It indicated no somaclonal variation during embryogenesis micropropagation